Quantitative Analysis of Ellagic Acid in Nymphaea tetragona Georgi Root by HPLC-UVD

Lee, Dong Young (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Jeong, Eun Ju (Department of Agronomy and Medicinal Plant Resources, College of Life Sciences and Natural Resources, Gyeongnam National University of Science and Technology)
Jeon, Byung Ju (Institute for Life Science, Elcomscience Co. Ltd.)
Sung, Sang Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)

published in 2014. 3. 31

Abstracts

A high performance liquid chromatography (HPLC) method for the quantitation of ellagic acid in Nymphaea tetragona was developed for the quality control of functional cosmetic ingredient, the extract of N. tetragona. Separation and quantitation were successfully achieved with a Kromasil C18 column ($5{\mu}m$, $250mm{\times}4.6mm$, i.d.) by isocratic elution of a mixture of acetonitrile containing 0.1% trifluoroacetic acid and water containing 0.03% phosphoric acid at a flow rate of 1.0 ml/min. The UV detector was used for the detection and the wavelength for quantitation was set at 254 nm. The presence of ellagic acid in the extract was determined by comparison of retention time and spiking with authentic standard. Analytical results showed good linearity ($R^2=0.99996$) in relatively wide concentration ranges. The R.S.D. for precision test was less than 3.0%. Recovery of the compound was 98.55~101.72% with R.S.D values less than 4.0%. In conclusion, this method has been successfully applied to the determination of ellagic acid in N. tetragona.

Keyword

Nymphaea tetragona
HPLC-UVD
Ellagic acid
Quantitative analysis

Chemical Compound (All 2)

Geraniin (C₄₁H₂₈O₂₇)

(2S,3S,4R,5R,6R)-3-[(2S,3R,5S,6R)-3-acetamido-4-[(2R,3R,4S,5S,6S)-6-carboxy-3,4,5-trihydroxy-oxan-2-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[(2R,3R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-4,5-dihydroxy-oxane-2-carboxylic acid (C₂₈H₄₄N₂O₂₃)

Analytical Information

Analytical Information of the Articles
IPC	A61K 125/00, A61K 31/352, A61K 36/62, G01N 30/02, G01N 30/74
Analytical Keyword	Nymphaea tetragona Georgi; ellagic acid; root; HPLC-UVD=high-performance liquid chromatographic method with UV detector; quantitative analysis; retention time; MeOH=methanol; extract
Herb keywrd	Nymphaea tetragona Georgi
Etc. keywrd	ellagic acid; root; HPLC-UVD=high-performance liquid chromatographic method with UV detector; quantitative analysis; retention time; MeOH=methanol; extract

Published Information

Cover Copyright

Published Information of the Articles
Country	kor
Publisher	The Korean Society of Pharmacognosy
KJTK Publish Information	Volume 8. 2014. 12. 31
TKOI It is persistent identifier of content provided by the Korean Traditional Knowledge Portal. It is used as a permanent access route to each content. Syntax: http://tkoi.koreantk.com/[TKOI] Example: http://tkoi.koreantk.com/TKOI:KTKP-TSS-0000045749	TKOI:KTKP-TSS-0000225631

Korean Journal of Pharmacognosy (생약학회지)