Optimal Extraction and Characteristics of Lignocellulytic Enzymes from Various Spent Mushroom Composts
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Lim, Sun Hwa
(Graduate School of Future Convergence Technology, Hankyong National University)
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Lee, Yun Hae
(Mushroom Research Institute, GARES)
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Kang, Hee Wan
(Graduate School of Future Convergence Technology, Hankyong National University)
published in 2013. 9. 30
Abstracts
Recovery of ${\alpha}$-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$-xylosidase (EC 3.2.1.37), ${\beta}$-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) from spent mushroom composts (SMCs) of Pleurotus cornucopiae, Pleurotus ostreatus, Pleurotus eryngii, Hericium erinaceum, Lyophyllum ulmarium, Agrocybe cylindracea, Lentinus lepideus, and Flammulina velvtipes were investigated using different extraction buffers. The maximum recovery of the enzymes was mostly detected in SMC extracts with tap water and 0.25% Triton X-100 by shaking incubation (200 rpm) for 2 h at $4^{\circ}C$. The xylanase (152 U/g) and laccase (8.1 U/g) activities were the highest in SMC extracts from F. velvtipes and P. eryngii. In addition, high enzymatic activities of ${\alpha}$-amylase (3.6 U/g) and cellulase (3.4 U/g) was detected in SMC extract of A. cylindracea. Futhermore, cellulase and laccase activities of SMCE from P. eryngii were compared to commercial enzymes.
Keyword
- Lignocellulytic enzymes
- Recovery
- Spent mushroom composts
Analytical Information
Analytical Information of the Articles
IPC |
A01G 18/00, C12N 9/42 |
Analytical Keyword |
Hericium erinaceum; Lyophyllum ulmarium; Pleurotus cornucopiae; Lentinus lepideus; Agrocybe cylindracea; lignocellulytic Enzymes; laccase; cellulase; amylase; lignin peroxidase; xylanase; beta-xylosidase; beta-glycosidase; spent mushroom composts
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Etc. keywrd |
Hericium erinaceum; Lyophyllum ulmarium; Pleurotus cornucopiae; Lentinus lepideus; Agrocybe cylindracea; lignocellulytic Enzymes; laccase; cellulase; amylase; lignin peroxidase; xylanase; beta-xylosidase; beta-glycosidase; spent mushroom composts
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Published Information of the Articles
Country |
kor |
Publisher |
The Korean Society of Mycology |
KJTK Publish Information |
Volume 8.
2014. 12. 31
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