Sodium hypochlorite treatment and light-emitting diode (LED) irradiation effect on in vitro germination of Oreorchis patens (Lindl.) Lindl

• Bae, Kee Hwa (National Institute of Biological Resources)
• Oh, Kyoung Hee (National Institute of Biological Resources)
• Kim, Soo-Young (National Institute of Biological Resources)

published in 2014. 3. 31

Abstracts

In this study, we investigated the effects of sodium hypochlorite (NaOCl) and red or blue light-emitting diode (LED) light on embryo swelling and germination of Oreorchis patens (Lindl.) Lindl. A method for determining the swelling and protocorm formation in O. patens seeds through in vitro examination of immature seeds was established. NaOCl treatment of immature seeds was found to significantly enhance the extent of embryo swelling and protocorm formation in immature zygote embryos compared to those in the untreated controls. Additionally, the effects of white fluorescent light, and red and blue LED lights on embryo swelling and protocorm formation in in vitro cultured seeds were examined and compared to the conditions with or without NaOCl treatment. The most suitable light for embryo swelling and protocorm formation was the red LED light.

Keyword

• Germination
• Medium
• Orchid
• Oreorchis patens

Published Information

Cover Copyright

Published Information of the Articles

Country	kor
Publisher	The Korean Society of Plant Biotechnology
KJTK Publish Information	Volume 8. 2014. 12. 31
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